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RNA Structure - Medical Science

Q1:

A nicked RNA molecule can be ligated by

A T4 RNA ligase

B DNA polymerase

C T4 DNA ligase

D all of these

ANS:C - T4 DNA ligase

Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids.