Protein Purification

Q1: A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

A 1,000 units/mg

B 10,000 units/mg.

C 100,000 units/mg

D 1,000,000 units/mg

ANS:C - 100,000 units/mg

1 unit has been defined in the problem as 1 millimole synthesised per second.

It clearly says 10 microgram protein has one unit activity. So Specific activity is I unit/ 10 microgram protein and therefore it is 100units/mg.

This alternative is not given. Why should we change the given definition of the unit of enzyme in the problem?

Agreed that international definition of a unit is different.



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