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Immunological Techniques - Medical Science

Q1:

An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

A there was more than one antigen

B the antibody was impure

C the temperature was too high

D there was one antibody and one antigen

ANS:A - there was more than one antigen

I agree there could be more than one antigen but also the specific antibodies suspended into the gel should also be more than one to enable binding to their specific antigens present. Lest I could argue that the results were invalid.