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Nucleic Acids

Q1: Because DNA is a highly charged polyanion, its stability to heat denaturation

A does not depend on hydrophobic interactions

B increases with increasing salt

C is independent of G - C content

D decreases with increasing salt

ANS:B - increases with increasing salt

Increase in salt concentration mask the charge on both strands of DNA. Thus making them very stable and the resultant repulsion force will be minimized. As a result the stability of DNA increases with increase in salt concentration and require higher temperature than in absence of salt.



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