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Protein Purification

Q1:

During successful purification scheme, this may be expected that the

A specific activity increases

B specific activity decreases

C number of proteins in the sample decreases

D both (a) and (c)

ANS:D - both (a) and (c)

No.of proteins should decrease. then only we will be left with the protein of interest.

Specific activity = protein activity/ total mg of protein present.

So as we proceed towards more purification steps, we see increase in specific activity.



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