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Gel Electrophoresis - Medical Science

Q1:

Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

A both proteins migrate to the anode

B histones migrate to the anode and myoglobin migrates to the cathode

C histones migrate to the cathode and myoglobin migrates to the anode

D both proteins migrate to the cathode

ANS:C - histones migrate to the cathode and myoglobin migrates to the anode

As compared to PI, Increased pH leads increased the H+ ion concentration, so it migrates towards -vely charged electrode (Cathode). When in decreased pH, increases the OH- ion concentrations, then it migrates towards +vely charged electrode (Anode).