Gel Electrophoresis - Medical Science

Q1:

In a native PAGE, proteins are separated on the basis of

A net negative charge

B net charge and size

C net positive charges size

D net positive charge

ANS:B - net charge and size

If two proteins have the same charges but differ in their molecular weight. Which of the protein will come out first in a native page?

Is it the smaller molecular weight or large molecular weight?