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Polymerase Chain Reaction

Q1: In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

A 16 single strands of DNA

B 16 double stranded DNA

C 18 single strands of DNA

D 18 double stranded DNA

ANS:A - 16 single strands of DNA

Each strand will be calculated as 2 raise to power n. n is four here therefore no of strands will be either two raise to power 4 = 16*2 (for one one mole of DNA).

32 single strands or 16 double strands.



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