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Gel Electrophoresis - Medical Science

Q1:

In an SDS-PAGE

A proteins are denatured by the SDS

B proteins have the same charge-to-mass ratio

C smaller proteins migrate more rapidly through the gel

D all of the above

ANS:D - all of the above

SDS is an ionic detergent which imparts the negative charge to denatured proteins, generally, one SDS molecule binds with two amino acids hence all proteins in the sample have a negative charge so that the protein in the gel will migrate on the basis of charge to mass ratio. The proteins having less molecular weight will migrate faster in the gel as compare to the proteins having the higher mass to charge ratio.