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Gel Electrophoresis - Medical Science

Q1:

In isoelectric focusing, proteins are separated on the basis of their

A relative content of positively charged residue only

B relative content of negatively charged residue only

C size

D relative content of positively and negatively charged residue

ANS:D - relative content of positively and negatively charged residue

Isoelectric focusing means the separation of the protein based on the isoelectric pH. Isoelectric pH is the pH at which the protein can exist at the neutral state.

For being neutral it should have both positive and negative charged residue. Thus isoelectric focusing is based on the relative content of positively and negatively charged residue.