Polymerase Chain Reaction - Medical Science

Q1:

Polymerase chain reaction basically consists of

A two steps

B three steps

C four steps

D five steps

ANS:B - three steps

Denaturation
Annealing
Extention.

Pneumonics for this is DAE.

The first is at 90 to 97 degree.
2nd step is 68degree.
3rd step is 72 degree.