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Gel Electrophoresis

Q1:

Proteins are separated in an SDS-PAGE experiment on the basis of their

A positively charged side chains

B molecular weight

C negatively charged side chains

D different isoelectric points

ANS:B - molecular weight

Let say we have a approximately 10ug of protein running in a gel, what would look like if we load 1ug of protein on top of it?



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