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Gel Electrophoresis - Medical Science

Q1:

Proteins can be visualized directly in gels by

A staining them with the dye

B using electron microscope only

C measuring their molecular weight

D none of these

ANS:A - staining them with the dye

The staining of the protein helps in the visualizing the protein in the form of the coloured band that also ease the identification of the protein and the isolation of the protein.

The staining of the protein can be done with the help of coomassie blu dye G250. This dye binds to the protein and thus help in the estimation of the protein. When DNA electrophoresis is been carried out the dye used is ethylene bromide.