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Polymerase Chain Reaction

Q1: RNA populations can also be used as PCR templates after reverse transcription into

A c DNA

B t RNA

C m DNA

D r RNA

ANS:A - c DNA

Reverse transcription(RT) -PCR is used to amplify RNA targets. The RNA template is converted into complementary(c) DNA by the enzyme reverse transcriptase.

The cDNA serves later as a template for exponential amplification using PCR.



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