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Recombinant DNA - Medical Science

Q1:

The advantage of using DNA polymerases from thermophilic organisms in PCR is that

A the DNA polymerases of these bacteria are much faster than those from other organisms

B the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

C the DNA polymerases of these bacteria never make mistakes while replicating DNA

D all of the above

ANS:B - the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

The polymerase chain reaction is a thermo-cycling process involving high temperature as 94°C, DNA polymerase of thermophilic bacteria can survive such temperature. An example is a commonly used Taq-polymerase enzyme isolated from Thermophylus aquatics.