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Protein Purification - Medical Science

Q1:

The best way to determine the location of protein in the purification scheme is to measure the

A rate of ATP synthesis

B changes in the refractive index

C UV absorption

D mass spectroscopy of the protein

ANS:A - rate of ATP synthesis

I think the answer must be D to know if there still any more impurities still present, but answer c is correct to determine concentration of protein especially when purified in gel electrophoresis.