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Animal Cell Culture and Regulation

Q1:

Toxicity due to accumulation of ammonia can be overcome

A by substituting glutamine by glutamate

B by controlled addition of glutamine at low level

C by removal of ammonia or ammonium from culture medium

D all of the above

ANS:D - all of the above

Yes, it lead to glutamine metabolism and ammonia in form alanine releases and decrease ammonia concentration.



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