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Immunological Techniques - Medical Science

Q1:

What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

A The ELISA would not develop when the substrate was added

B The ELISA would develop normally

C All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

D None of the above

ANS:C - All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

No answer description is available.