Gene Transfer in Plants - Medical Science

Q1:

Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

A It is carried out with the help of micromanipulator

B The recipient cells are immobilized on artificial support or artificially bound to substarate

C It employs needle with diameter greater than cell diameter

D all of the above

ANS:C - It employs needle with diameter greater than cell diameter

Give reason. How it will be?