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Recombinant DNA

Q1: Which type of restriction endonuclease cuts the DNA within the recognition site?

A Type I

B Type II

C Type III

D All of the these

ANS:B - Type II

Type I enzymes cleave at sites remote from a recognition site.

Type II enzymes cleave within or at short specific distances from a recognition site. The most common Type II enzymes are HhaI, HindIII, and NotI, that cleave DNA within their recognition sequences.

Type III enzymes cleave at sites a short distance from a recognition site.

Type IV enzymes target modified DNA, Eg. Methylated, hydroxymethylated and glucosyl hydroxymethylated DNA.



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