Cell Cultures and Characteristics

Cell Cultures and Characteristics

Q1: The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as

A selective medium

B enrichment medium

C differential medium

D none of these

Q2: In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

A -60 to -78°C

B -20 to -30°C

C -30 to -48°C

D -48 to -58°C

Q3: Viable plate count can be obtained using

A Spread plate method

B Pour plate method

C both (a) and (b)

D Hemocytometer

Q4: Which of the following problem makes it impossible to satisfy all of Koch's postulates?

A Microorganism causes serious symptoms in humans

B Microorganism cannot be isolated in pure culture

C Species of microorganism is in question

D Genes from the microorganism cannot be amplified by PCR

Q5: Plate counts are often reported as

A cfu

B cfu/ml

C Both (a) and (b)

D thousands/ml

Q6: Which of the following bacteria can grow in alkaline pH?

A Shigella

B Lactobacilli

C Salmonella

D Vibrio cholerae

Q7: Agar is used for solidifying culture media because

A it does not affect by the growth of bacteria

B it does not add to the nutritive properties of the medium

C the melting and solidifying points of agar solution are not the same

D all of the above

Q8: Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

A coliform bacteria

B gram positive bacteria

C yeasts

D acid fast organisms

Q9: The solidifying agent commonly used in preparation of media is/are

A agar

B silica gel

C both (a) and (b)

D none of these

Q10: Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

A bile salts

B low concentration of various dyes

C sugars

D low levels of nitrogen

Q11: When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria, it is known as

A differential medium

B enriched medium

C enrichment medium

D selective medium

Q12: The concentration of agar to obtain semi solid media is

A 1.5-20%

B 0.5% or less

C >10%

D >10%but<20%

Q13: A medium containing crystal violet dye plus sodium deoxycholate will allow

A gram (-)ve intestinal bacteria to grow

B gram (+)ve intestinal bacteria to grow

C aquatic bacteria to grow

D none of these

Q14: A disease producing species occurring in a mixed culture can often be selected on the basis of its

A pathogenic properties

B special carbon source

C special nitrogen source

D none of these

Q15: Which of the following bacteria dies quickly after drying?

A Treponema pallidium

B Mycobacterium tuberculosis

C Staphylococcus aureus

D Pseudomonas aeruginosa

Q16: Cell counting can be carried out by

A direct microscopic count using petroffhansser counting chamber

B plate counting

C membrane filter count

D all of the above

Q17: For the selection of lactobacilli present in cheddar cheese, the pH of the medium is maintained at

A 6.35

B 5.35

C 4.35

D 5.75

Q18: In the pour plate method, the mixed culture is diluted directly in tubes of

A liquid agar medium

B sterile liquid usually water

C both (a) and (b)

D none of these

Q19: Which of the following chemotherapeutic agents may affect the growth of Campylobacter jejuni?

A Vacomycin

B Polymyxin

C Trimethoprim

D None of these

Q20: Which of the following bacteria can grow in acidic pH?

A Vibrio cholerae

B Lactobacilli

C Shigella

D Salmonella

Q21: Which of the following is used for the proper maintenance and preservation of pure cultures?

A Periodic transfer to fresh media

B Preservation by overlaying cultures with mineral oil

C Preservation by lyophilization

D All of the above

Q22: Which of the following methods is most likely to be quantitative?

A Dilution and plating

B Gram staining

C Wet mount

D None of these

Q23: Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

A Pour plate

B Spread plate

C Both (a) and (b)

D Streak plate technique

Q24: Mac-Conkey medium is an example of

A transport medium

B enrichment medium

C differential medium

D all of these

Q25: Cell size and cell number can be measured using

A hemocytometer

B coulter counter

C petroff-hausser counting chamber

D none of these

Q26: Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria?

A Gas in specimen

B Foul odour

C Presence of sulphur granules

D All of these

Q27: Important example(s) of enriched medium/media is/ are

A Loeffler's serum slope

B Bordet-gengou medium

C Blood agar

D All of these

Q28: When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits the growth of wanted bacteria, it is known as

A differential medium

B enriched medium

C enrichment medium

D selective medium

Q29: For the selection of endospore-forming bacteria, a mixed culture can be heated at

A 80°C for 10 minutes

B 70°C for 10 minutes

C 60°C for 10 minutes

D 90°C for 10 minutes

Q30: To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

A 8.5

B 8.90

C 6.79

D 3.5

Q31: All bacteria that inhabit the human body are

A heterotrophs

B chemolithotrophs

C autotrophs

D phototroph

Q32: Stuart's transport medium is used for transport of specimen containing

A Neisseria gonorrhoeae

B Salmonella

C Vibrio cholerae

D Shigella

Q33: Treponema species from human oral cavity can be selected by using

A pH of the medium

B incubation temperature

C cell size and motility

D all of these

Q34: Peptone water and nutrient broth are

A selective media

B enriched media

C basal media

D none of these

Q35: Pseudomonas aeruginosa forms a blue water soluble pigment called

A pyocyanin

B chlororaphin

C pyoverdin

D β-carotene

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