DNA Structure and Replication

Q1: DNA gyrase in E. coli

A performs the same function as helicase in eukaryotes

B is required only at the oriC site

C adds positive supercoils to chromosomal DNA

D can be inhibited with antibiotics

ANS:B - can be inhibited with antibiotics

DNA gyrase in E.coli relaxes the tension in the strand that builds ahead of the replication fork during replication. If this fails to occur, replication will cease and the cell will die. This is employed by anti biotics to inhibit replication of bacteria such as E.Coli.

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