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DNA Structure and Replication

Q1: Which of the following is used to determine the appropriate stringency (Wallace rule)?

A Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

B Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

C Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

D Tm = 69.3° + 0.41 (%)G+C -650/1

ANS:C - Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

No answer description is available.



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